Protocols

Protein expression and purification

NBD Expression and Purification Protocol
General protocol for the expression and purification of Smt3-tagged NBD proteins using Ni-NTA and GFC approaches from the Thibodeau lab. May need to optimized for specific NBD proteins and variants.
NBD Protein Expression and Purification.[...]
Adobe Acrobat document [55.2 KB]

Protein analysis

Thermofluor stability assay
General protocol for the SYPRO orange thermofluor thermal stability assay.
Thermofluor Assay using SYPRO Orange.pdf
Adobe Acrobat document [69.3 KB]
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© 2017 CFTR 3D-Structure Consortium; Updated July 25, 2017